Premium
Delftia tsuruhatensis WGR–UOM–BT1, a novel rhizobacterium with PGPR properties from Rauwolfia serpentina (L.) Benth. ex Kurz also suppresses fungal phytopathogens by producing a new antibiotic—AMTM
Author(s) -
Prasannakumar S.P.,
Gowtham H.G.,
Hariprasad P.,
Shivaprasad K.,
Niranjana S.R.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12479
Subject(s) - rhizosphere , biology , antimicrobial , alternaria solani , strain (injury) , microbiology and biotechnology , horticulture , botany , food science , bacteria , fungicide , genetics , anatomy
The bacterial strain designated as WGR – UOM – BT 1 isolated from rhizosphere of Rauwolfia serpentina exhibited broad‐spectrum antifungal activity and also improved early plant growth. Based on morphological, biochemical and 16S rRNA gene sequence analyses, the strain BT 1 was identified as Delftia tsuruhatensis (KF727978). Under in vitro conditions, the strain BT 1 suppressed the growth of wide range of fungal phytopathogens. Purified antimicrobial metabolite from the strain BT 1 was identified as nitrogen‐containing heterocyclic compound, ‘amino(5‐(4‐methoxyphenyl)‐2‐methyl‐2‐(thiophen‐2‐yl)‐2,3‐dihydrofuran‐3‐yl)methanol’ ( AMTM ), with molecular mass of 340•40 and molecular formula of C 17 H 19 NO 3 S. The strain BT 1 was positive for rhizosphere colonization (tomato), IAA production, 1‐aminocyclopropane‐1‐carboxylate (ACC) deaminase activity and phosphate solubilization. Under laboratory and greenhouse conditions, the strain BT 1 promoted plant growth and suppressed foliar and root fungal pathogens of tomato. Therefore, antimicrobial and disease protection properties of strain BT 1 could serve as an effective biological control candidate against devastating fungal pathogens of vegetable plants. Besides, the production of IAA , P solubilization and ACC deaminase activity enhance its potential as a biofertilizer and may stabilize the plant performance under fluctuating environmental conditions. Significance and Impact of the Study In this study, we reported that Delftia tsuruhatensis WGR – UOM – BT 1 strain has the plant growth promotion activities such as rhizosphere colonization (tomato), IAA production, 1‐aminocyclopropane‐1‐carboxylate (ACC) deaminase activity and phosphate solubilization. This bacterial strain was found producing an antimicrobial nitrogen‐containing heterocyclic compound identified as ‘amino(5‐(4‐methoxyphenyl)‐2‐methyl‐2‐(thiophen‐2‐yl)‐2,3‐dihydrofuran‐3‐yl)methanol’ [C 17 H 19 NO 3 S] ( AMTM ), which is new to the bacterial world.