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Improvement of ( R )‐3‐hydroxybutyric acid secretion during Halomonas sp. KM ‐1 cultivation with saccharified Japanese cedar by the addition of urea
Author(s) -
Kawata Y.,
Nojiri M.,
Matsushita I.,
Tsubota J.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12473
Subject(s) - halomonas , intracellular , fermentation , extracellular , food science , secretion , chemistry , biochemistry , bacteria , microbiology and biotechnology , biology , botany , halophile , genetics
Japanese cedar ( Cryptomeria japonica ) is a major species in artificial Japanese forests. The Halomonas sp. KM ‐1 was recently isolated and found to grow effectively on saccharified Japanese cedar wood, resulting in the intracellular storage of poly‐( R )‐3‐hydroxybutyric acid ( PHB ) under aerobic conditions. Under microaerobic conditions, the extracellular secretion of ( R )‐3‐hydroxybutyric acid (( R )‐3‐ HB ) led to the degradation of intracellular PHB . In this study, the production of PHB and the secretion of ( R )‐3‐ HB using saccharified Japanese cedar were much improved in cultures that were grown in the presence of urea. The level of intracellular PHB production after 36 h under aerobic cultivation was 23·6 g l −1 ; after shifting to microaerobic conditions for 24 h, the ( R )‐3‐ HB concentration in the medium reached 21·1 g l −1 . Thus, KM ‐1 efficiently utilizes saccharified Japanese cedar to produce PHB and secretes ( R )‐3‐ HB , making it a practical candidate for use in the industrial production of ( R )‐3‐ HB . Significance and Impact of the Study Japanese cedar is a major species grown in artificial Japanese forests, and its thinning is crucial for the health of artificial forests and the Japanese economy. Halomonas sp. KM ‐1 grew effectively on saccharified Japanese cedar wood, resulting in intracellular storage of poly‐( R )‐3‐hydroxybutyric acid ( PHB ) under aerobic conditions. Under microaerobic conditions, extracellular secretion of ( R )‐3‐hydroxybutyric acid (( R )‐3‐ HB ) caused intracellular PHB degradation. ( R )‐3‐ HB is a chiral compound that is useful in the chemical, health food and pharmaceutical industries. The production of PHB and secretion of ( R )‐3‐ HB using saccharified wood was dramatically improved, which may positively affect its future industrial production.