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Characterization of L isteria monocytogenes from three countries and antibiotic resistance differences among countries and L isteria monocytogenes serogroups
Author(s) -
Obaidat M.M.,
Bani Salman A.E.,
Lafi S.Q.,
AlAbboodi A.R.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12420
Subject(s) - microbiology and biotechnology , clavulanic acid , tetracycline , penicillin , clindamycin , amoxicillin , listeria monocytogenes , erythromycin , biology , antibiotic resistance , ampicillin , antibiotics , bacteria , genetics
A total of 104 Listeria monocytogenes isolates from 330 fish samples from three countries were characterized by multiplex PCR for serogrouping and virulence markers determination and tested for antibiotics resistance. A 53·8% of the isolates belonged to serogroup 1/2a, 3a; 32% belonged to 1/2b, 3b, 7; 14·4% belonged to 4b, 4d, 4e and 1% belonged to 1/2c, 3c. All isolates exhibited resistance to at least one antibiotic but the resistance rates varied among countries. The isolates exhibited high resistance to penicillin, rifampicin, clindamycin, erythromycin and tetracycline, but low resistance to amoxicillin‐clavulanic acid, streptomycin, sulfamethoxazole–trimethoprim, gentamicin, chloramphenicol and kanamycin. When comparing countries, the resistance rate for rifampicin, clindamycin, erythromycin, tetracycline, amoxicillin‐clavulanic acid varied among countries. When comparing serogroup, 1/2a, 3a exhibited the highest resistance to clindamycin, erythromycin, tetracycline and vancomycin while serogroup 4b, 4d, 4e exhibited the highest resistance to amoxicillin‐clavulanic acid. All isolates carried inlA , inlC , inlJ and lmo 2672. Listeriolysin S was carried by 42 and 30% of 4b and 1/2b isolates respectively. Significance and Impact of the Study This is one of few studies to correlate antibiotic resistance with Listeria monocytogenes serogroups. The study also compared the antibiotic resistance and serogroups of L. monocytogenes isolates from three countries in one single study. The findings of this study will be helpful in improving data on the antibiotics resistance of L . monocytogenes in developing countries and enriches the epidemiological and public health studies of L. monocytogenes .