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Isolation and characterization of B acillus subtilis CH 16 strain from chicken gastrointestinal tracts for use as a feed supplement to promote weight gain in broilers
Author(s) -
Nguyen A.T.V.,
Nguyen D.V.,
Tran M.T.,
Nguyen L.T.,
Nguyen A.H.,
Phan T.N.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12411
Subject(s) - bacillus subtilis , bacillus licheniformis , feed conversion ratio , biology , spore , weight gain , broiler , strain (injury) , bacillus (shape) , food science , microbiology and biotechnology , bacillales , zoology , bacteria , body weight , genetics , anatomy , endocrinology
Spore‐forming bacterial strains were isolated from chicken gastrointestinal tracts to develop a heat‐stable feed supplement that promotes weight gain in broilers. Seven Bacillus strains having more than 90% sporulation were screened from the isolates and identified to be closely related with Bacillus subtilis and Bacillus licheniformis . Of the seven strains, B. subtilis CH 16 was selected to develop a feed supplement for broilers, because it formed 100% heat‐stable spores, grew rapidly at 42°C and quickly formed a biofilm. In large‐scale trials in broilers ( n ≥ 1150 per group), the group fed CH 16 (3 × 10 6 CFU g −1 pellet) showed higher average daily gain ( ADG = 61·16) and lower food conversion ratio ( FCR = 1·696) than did the group fed B. licheniformis CH 22 ( ADG = 57·10 and FCR = 1·792), the group fed B. subtilis HU 58 ( ADG = 51·90 and FCR = 1·868), BioPlus group ( ADG = 59·32 and FCR = 1·807) and the control group ( ADG = 56·02 and FCR = 1·880). In conclusion, CH 16 spores significantly increased ADG by 9·17% and reduced FCR by 9·79% in broilers. The result supports the use of B. subtilis CH 16 of chicken intestinal origin as a feed supplement that promote weight gain in broilers. Significance and Impact of the Study This study reports screening of Bacillus strains isolated from chicken gastrointestinal tracts for development of a feed supplement that promote weight gain in broilers. Of the seven Bacillus isolates with high sporulation efficiency (≥90%), Bacillus subtilis CH 16 strain showed the best growth and biofilm formation at body temperature of broilers (42°C). In large‐scale trials in broilers ( n ≥ 1150 per group), CH 16 spores induced a 9·17% increase in daily weight gain ( ADG ) and a 9·79% reduction in FCR while the commercial BioPlus ® YC induced only a 5·89% increase in ADG and a 3·88% reduction in FCR .