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Phylogenetic diversity of sulphate‐reducing D esulfovibrio associated with three S outh C hina S ea sponges
Author(s) -
Zhang D.,
Sun W.,
Feng G.,
Zhang F.,
Anbuchezhian R.,
Li Z.,
Jiang Q.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12400
Subject(s) - desulfovibrio , biology , deltaproteobacteria , phylogenetic diversity , sponge , phylogenetic tree , sulfate reducing bacteria , 16s ribosomal rna , phylogenetics , bacteria , zoology , ecology , botany , genetics , gammaproteobacteria , gene
Marine sponges harbour dense and diverse micro‐organisms which includes sulphate‐reducing bacteria ( SRB ). SRB are known to play a key role in the cycling of marine elements. However, in contrast to carbon and nitrogen cycling bacteria, SRB associated with marine sponges are largely unexplored. In this study, we explored the phylogenetic diversity of the SRB associated with three shallow‐water sponges Arenosclera heroni , Dysidea arenaria and Astrosclera willeyana from the South China Sea by cloning‐and‐sequencing approach of SRB 16S rRNA gene with specific primers. The results showed that SRB associated with sponges mainly belonged to the genus Desulfovibrio in the class Deltaproteobacteria, i.e. a total of 14 Desulfovibrio ‐related OTU s were obtained from three sponges. The exception is identical OTU s from different sponges. Each sponge species harboured a unique set of Desulfovibrio OTU s, with only a few shared OTU s observed between species, suggesting different species of Desulfovibrio in different species of sponges. Meanwhile, some Desulfovibrio OTU s had a low similarity (<97%) with related sequences in GenBank and phylogenetic analysis indicating novel Desulfovibrio symbionts in sponges. The results contribute to the overall understanding of the phylogenetic diversity of SRB associated with sponges. Significance and Impact of the Study To date, in contrast to carbon and nitrogen cycling bacteria, sulphate‐reducing bacteria ( SRB ) associated with marine sponges are largely unexplored; little is known about the phylogenetic diversity of SRB in different species of sponges. In the present study, phylogenetically diverse sulphate‐reducing Desulfovibrio communities, including potential sponge species‐specific and novel SRB , were revealed to be associated with South China Sea demosponges by cloning‐and‐sequencing approach of SRB 16S rRNA gene.