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Evaluation of a biofilm formation by D esulfovibrio fairfieldensis on titanium implants
Author(s) -
Jorand F.P.A.,
Debuy S.,
Kamagate S.F.,
EngelsDeutsch M.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12370
Subject(s) - biofilm , titanium , materials science , microbiology and biotechnology , chemistry , bacteria , biology , metallurgy , genetics
The aim of this study was to assess the capabilities of D esulfovibrio fairfieldensis to colonize the grade 4 titanium coupons ( mod SLA ) used in dental implants. The effect of ampicillin, which is known to be a poorly penetrating agent in the matrix biofilm, was also compared with planktonic and sessile cells. The mod SLA colonization by bacteria in KNO 3 (0·05 mol l −1 ) and culture media ( DSM 63 and fetal bovine serum) was determined by direct cell counts and field emission electronic microscopy. The surface of titanium (Ti) coupons was characterized by scanning electron microscopy and by Raman spectroscopy. Cells, mainly located in surface pores of mod SLA coupons, appeared to be wrapped in a polymeric‐like structure. The initial apparent rates of adhesion varied from 3 × 10 6 to 30 × 10 6 cells cm −2 h −1 , and a plateau was reached at 1 day, regardless of the incubation medium. No cells have significantly adhered to polished Ti, and a minority was found on massive Ti. Finally, cells trapped on the mod SLA surface were not lysed by ampicillin contrary to planktonic cells. Des. fairfieldensis is therefore able to colonize the rough surface of mod SLA implant through a physical trapping in the microporosity of the surface, where they can produce a biofilm‐like structure to improve their resistance to ampicillin. Significance and Impact of the Study D esulfovibrio fairfieldensis is one of the most relevant sulphate‐reducing bacteria of the human oral cavity suspected to be involved in peri‐implantitis and implant corrosion. This study demonstrates for the first time that Des. fairfieldensis is able to initiate the formation of a biofilm‐like structure on the microstructured titanium coupons used in dental implants and that it improves its resistance to antibiotic treatment. It gives new insight to understand the capacity of this opportunistic pathogen to colonize implant surfaces and to resist to biocide treatments.