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Conjugative reporter system for the use in Bacillus licheniformis and closely related Bacilli
Author(s) -
Hertel R.,
Volland S.,
Liesegang H.
Publication year - 2015
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12352
Subject(s) - bacillus licheniformis , bacilli , bacillales , bacillaceae , microbiology and biotechnology , bacillus (shape) , bacteria , biology , bacillus subtilis , genetics
Abstract Bacillus wild‐type strains are genetically difficult to manipulate, and thus, the options for rational strain investigation and design are limited. Here, we present a set of small conjugative shuttle vectors for the use in Bacillus licheniformis and related, genetically difficult accessible wild‐type strains. The vector set comprises the modular general‐purpose vector pV 2 and its derivatives pV 3SDlacZ and pV 3lacZ. The pV 3 vectors are designed for the investigation of transcriptional and translational activities of regulatory regions like promoters and ribosomal binding sites (RBS). The vector set has been tested for investigating gene regulation under aerobic and anaerobic conditions. Significance and Impact of the Study The hereby introduced vectors represent a novel easy to apply toolbox which makes Bacillus strains with low or without natural competences accessible through transconjugation and allows quantitative studies on gene activity and gene regulation. Thus, the tool set enables research on newly isolated wild‐type strains with methods normally limited to laboratory strains.