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Development of an indirect ELISA with epitope on nonstructural protein of Muscovy duck parvovirus for differentiating between infected and vaccinated Muscovy ducks
Author(s) -
Yan B.,
Ma J.Z.,
Yu T.F.,
Shao S.L.,
Li M.,
Fan X.D.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12323
Subject(s) - virology , epitope , biology , parvovirus , virus , porcine parvovirus , canine parvovirus , antibody , immunology
The aim of this study was to develop an indirect enzyme‐linked immunosorbent assay (i‐ ELISA ) based on epitope AA 503‐509 ( RANEPKE ), which is on nonstructural protein of Muscovy duck parvovirus ( MDPV ). Sera (100) from negative and vaccinated Muscovy ducks were compared with infected sera (240) to establish the cut‐off value of this i‐ ELISA . There was a significant difference between the positive and negative populations ( P < 0·05). The adoption of this positive–negative threshold value for this i‐ ELISA assay resulted in specificity of 98·0%. This i‐ ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus. Significance and Impact of the Study In this study, we developed an i‐ELISA based on epitope AA503‐509 ( RANEPKE ), which is on nonstructural protein of MDPV. This i‐ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus.