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Detection of Co HV ‐1 by loop‐mediated amplification ( LAMP ). Application of LAMP for Co HV ‐1 incidence monitoring in domestic pigeons
Author(s) -
Woźniakowski G.,
Wencel P.,
SamorekSalamonowicz E.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12317
Subject(s) - loop mediated isothermal amplification , veterinary medicine , incidence (geometry) , virus isolation , biology , virology , medicine , virus , dna , physics , optics , genetics
The occurrence of Columbid herpesvirus‐1 (Co HV ‐1) in domestic pigeons presents a potential threat for their husbandry and trade. Co HV ‐1 causes Smadel disease among pigeons but may not be manifested by any clinical signs and complicates secondary infections. The material for our study originated from 42 domestic pigeons sent to private veterinary practice in Lublin, Poland, between 2011 and 2013. Some of birds showed clinical signs similar to Smadel disease. The cytological examination also indicated on Co HV ‐1 infection. The incidence of Co HV ‐1 was tested in DNA extracted from liver of birds by loop‐mediated isothermal amplification ( LAMP ). LAMP was used for the monitoring of Co HV ‐1 presence among pigeons in Poland. Our study showed that LAMP was capable of detecting Co HV ‐1 presence in 8 (19%) of 42 examined birds without the use of any advanced laboratory equipment. The results were confirmed by real‐time PCR and virus isolation in chicken embryo fibroblasts. This is the first report on LAMP application for successful detection of Co HV ‐1 in domestic pigeons. Significance and Impact of the Study The incidence of Columbid herpesvirus‐1 (Co HV ‐1) in pigeons was examined for the first time by loop‐mediated isothermal amplification ( LAMP ). The study showed the presence of Co HV ‐1 in 8 of 42 examined domestic pigeons. LAMP technique developed within this study may be used by not well‐equipped veterinary laboratories.