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Phenotypic and genotypic characterization of H 2 S‐positive and H 2 S‐negative strains of S hewanella baltica isolated from spoiled whiting ( M erlangius merlangus )
Author(s) -
Dehaut A.,
MideletBourdin G.,
Brisabois A.,
Duflos G.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12312
Subject(s) - shewanella , biology , baltica , whiting , shewanella putrefaciens , trimethylamine , microbiology and biotechnology , thermolabile , bacteria , genetics , biochemistry , enzyme , paleontology , ordovician , fishery , fish <actinopterygii>
Four strains were isolated from a spoiled whiting ( M erlangius merlangus ). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N ‐Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species but showed that one of the four strains was unable to produce H 2 S. Two strains synthetized an ornithine decarboxylase being potential putrescine producers. Results of carbon source use highlighted that the four strains were able to use citrate and d ‐sucrose and one strain was not able to use l ‐arabinose. Genotypic characterization of the strains thanks to 16S rRNA and gyrB partial gene sequencing led to their identification as members of S hewanella baltica species. These observations suggest that H 2 S production may not be the most appropriate screening parameter for S hewanella species and further to monitor the development of spoilage flora. Significance and Impact of the Study S hewanella is a complex genus composed of numerous and heterogeneous species. One of them S hewanella baltica has previously been described as one of the most important H 2 S‐producing bacterial species in iced stored fish and may act as spoilage organism through the reduction of trimethylamine N ‐Oxide (TMAO). Four strains of S. baltica were isolated from spoiled whiting ( M erlangius merlangus ), and description of three H 2 S‐positive strains and one H 2 S‐negative strain of S. baltica is highlighted in this short paper. Consequently, H 2 S production might not be the most appropriate screening parameter to assess the development of spoilage organisms.