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Isolation of a new broad spectrum antifungal polyene from Streptomyces sp. MTCC 5680
Author(s) -
Vartak A.,
Mutalik V.,
Parab R.R.,
Shanbhag P.,
Bhave S.,
Mishra P.D.,
Mahajan G.B.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12229
Subject(s) - microbiology and biotechnology , aspergillus fumigatus , candida krusei , biology , candida glabrata , polyene , candida albicans , streptomyces , cryptococcus neoformans , bacteria , corpus albicans , biochemistry , genetics
A new polyene macrolide antibiotic PN 00053 was isolated from the fermentation broth of Streptomyces sp. wild‐type strain MTCC ‐5680. The producer strain was isolated from fertile mountain soil of N aldehra region, H imachal P radesh, I ndia. The compound PN 00053 was purified through various steps of chromatographic techniques and bio‐activity guided fractionation followed by its characterization using physiochemical properties, spectral data ( 1 H‐ NMR , 13 C‐ NMR , HMBC , HSQC , and COSY ) and MS analysis. PN 00053 exhibited broad spectrum in vitro antifungal activity against strains of A spergillus fumigatus ( HMR ), A . fumigatus ATCC 16424, C andida albicans ( I . V .), C . albicans ATCC 14503, C . krusei GO 6, C . glabrata HO 4, C ryptococcus neoformans , T richophyton sp. as well as fluconazole resistant strains C . krusei GO 3 and C . glabrata HO 5. It did not inhibit growth of gram positive and gram‐negative bacteria, displaying its specificity against fungi. Significance and Impact of the Study PN 00053 is a novel polyene macrolide isolated from a wild strain of S treptomyces sp. PM0727240 (MTCC5680), an isolate from the mountainous rocky regions of H imachal P radesh, I ndia. The compound is a new derivative of the antibiotic R oflamycoin [32, 33–didehydroroflamycoin ( DDHR )]. It displayed broad spectrum antifungal activity against yeast and filamentous fungi. However, it did not show any antibacterial activity. The in vitro study revealed that PN00053 has better potency as compared to clinical gold standard fluconazole. The development of pathogenic resistance against the polyenes has been seldom reported. Hence, we envisage PN00053 could be a potential antifungal lead.