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Antimicrobial resistance and toxin gene profiles of S taphylococcus aureus strains from H olstein milk
Author(s) -
Wang X.,
Wang X.,
Wang Y.,
Guo G.,
Usman T.,
Hao D.,
Tang X.,
Zhang Y.,
Yu Y.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12221
Subject(s) - china , beijing , christian ministry , agriculture , microbiology and biotechnology , library science , biology , political science , ecology , law , computer science
Isolation of S taphylococcus aureus ( S taph. aureus ) from Holstein milk samples with mastitis and nonmastitis was conducted to estimate its prevalence, antimicrobial resistance and toxin genes. A total of 353 milk samples were collected from three C hinese H olstein herds. Fifty‐three S taph. aureus isolates collected from 29 S taph. aureus ‐positive samples were characterized via antimicrobial susceptibility, toxin genes and P ulsed‐field G el E lectrophoresis ( PFGE ) profiles. The prevalence of S taph. aureus was 4·0–9·5% in mastitic and 7·3–11·5% in nonmastitic samples in the analysed herds. Approximately 61·0% of S taph. aureus strains isolated from mastitis cows were resistant to ≥10 antimicrobials compared with 0% of isolates with nonmastitis. The most frequently observed super antigenic toxin gene was pvl (41·5%) followed by seh + pvl (13·2%). We did not find mecA ‐positive methicillin‐resistant Staph. aureus ( MRSA ) strains, while mecA ‐negative MRSA strains were identified in the three herds. PFGE results suggested potential transmission of S taph. aureus strains in different farms. These results open new insights into S taph. aureus transmission and antimicrobial resistance of Holstein dairy cows and into developing strategies for udder health improvement of dairy cattle.