Premium
Biofilm mediates E nterococcus faecalis adhesion, invasion and survival into bovine mammary epithelial cells
Author(s) -
Elhadidy M.,
Zahran E.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12184
Subject(s) - biofilm , enterococcus faecalis , microbiology and biotechnology , mastitis , biology , colonization , bacteria , pathogenesis , epithelium , mammary gland , staphylococcus aureus , immunology , genetics , cancer , breast cancer
We proposed in this study that during intramammary infection, biofilm formation may facilitate adherence and colonization of E nterococcus faecalis to mammary gland epithelium. This was established by comparing six different Ent. faecalis isolates with different biofilm‐forming profiles for their adhesive, invasive and survival capabilities to bovine mammary epithelial cell line ( MAC ‐ T ). Our results showed increased ability of the biofilm‐producer Ent. faecalis strains to adhere, invade and survive inside MAC ‐ T cells rather than nonbiofilm‐producer strains. We showed that growth of bacteria in bovine milk significantly augmented the adherence and invasion of all tested strains, and this feature was abolished again when strains were subcultured in brain heart infusion broth. Moreover, growth in bovine milk significantly increased biofilm formation by all tested strains. These results indicated that biofilm formation by E nt. faecalis , especially after expressing milk‐dependent induction, may have special relevance in the pathogenesis of E nt. faecalis mastitis during intramammary infection by enhancing bovine mammary epithelial adhesion and colonization. Significance and Impact of the Study Results obtained from current work highlighted the role of biofilm in the pathogenesis of E nterococcus faecalis mastitis. Those biofilm‐forming strains might be substantial as useful antigens in diagnostic assays and as future vaccine candidates to control E nt. faecalis mastitis.