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Isolation and characterization of broad spectrum bacteriophages lytic to V ibrio harveyi from shrimp farms of K erala, I ndia
Author(s) -
Surekhamol I.S.,
Deepa G.D.,
Somnath Pai S.,
Sreelakshmi B.,
Varghese S.,
Bright Singh I.S.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12175
Subject(s) - vibrio harveyi , biology , microbiology and biotechnology , shrimp , vibrio , aquaculture , lytic cycle , siphoviridae , phage therapy , myoviridae , bacteriophage , penaeus monodon , vibrio infections , isolation (microbiology) , bacteria , virology , fishery , virus , genetics , escherichia coli , gene , fish <actinopterygii>
Of 33 phages isolated from various shrimp farms in K erala, I ndia, six were segregated to have broad spectrum lytic efficiency towards 87 isolates of V ibrio harveyi with cross‐infecting potential to a few other important aquaculture pathogens. They were further tested on beneficial aquaculture micro‐organisms such as probiotics and nitrifying bacterial consortia and proved to be noninfective. Morphological characterization by transmission electron microscopy ( TEM ) and molecular characterization by RAPD and SDS ‐ PAGE proved them distinct and positioned under C audovirales belonging to M yoviridae and S iphoviridae. Significance and Impact of the Study In sustainable aquaculture, application of antibiotics is prohibited to manage vibriosis, including the one caused by V ibrio harveyi . In lieu of antibiotics, an eco‐friendly alternative method, phage therapy, is recommended here. To facilitate the same, a set of six broad spectrum V . harveyi phages, as cocktail, has been constituted and characterized based on morphological traits and by employing molecular tools. These phages were also found to infect other aquaculture pathogens belonging to V ibrio and A eromonas . Subsequent to in vivo trials, they can find application in shrimp hatcheries as prophylactics and therapeutics.