Premium
Diversity of cultivable β ‐glycosidase‐producing micro‐organisms isolated from the soil of a ginseng field and their ginsenosides‐hydrolysing activity
Author(s) -
Fu Y.,
Yin Z.,
Wu L.,
Yin C.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12166
Subject(s) - ginseng , glycoside hydrolase , biology , botany , diversity (politics) , microbiology and biotechnology , enzyme , biochemistry , medicine , alternative medicine , pathology , sociology , anthropology
This research aimed to explore the diversity of cultivable β‐glycosidase‐producing micro‐organisms in ginseng field soil. Fifty‐three strains showing β‐glucosidase activity were isolated from a ginseng field, using a newly designed E sculin‐ R 2A agar. All the isolated strains belonged to the genus Agrobacterium , A rthrobacter , B urkholderia , D yella , E daphobacter , L uteibacter , M ucilaginibacter , P aenibacillus , P henylobacterium , P seudomonas , S phingomonas and S treptomyces . The main β‐glucosidase‐producing micro‐organisms in the ginseng field soil were S phingomonas , B urkholderia , L uteibacter and S treptomyces , while concentrations of A grobacterium , A rthrobacter , P aenibacillus and P seudomonas were relatively low. Of these micro‐organisms, the strain GS 09 could hydrolyse major ginsenosides R b1, R b2 and R c to the active metabolite compound K . The strain GS 09 belonged to the genus S phingomonas, and its 16 S r RNA gene sequence showed 100% similarities with that of S phingomonas asaccharolytica . Significance and Impact of the Study This is the first study to provide information of cultivable β‐glycosidase‐producing micro‐organisms in ginseng field soil. The strain GS 09 has potential to be applied on the preparation for minor ginsenoside C ‐ K in pharmaceutical industry.