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Oral administration of L actobacillus brevis KB 290 to mice alleviates clinical symptoms following influenza virus infection
Author(s) -
Waki N.,
Yajima N.,
Suganuma H.,
Buddle B.M.,
Luo D.,
Heiser A.,
Zheng T.
Publication year - 2014
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12160
Subject(s) - virus , oral administration , immunology , bronchoalveolar lavage , influenza a virus , biology , microbiology and biotechnology , interferon , nasal administration , orthomyxoviridae , immunity , medicine , virology , immune system , lung , pharmacology
AbstractL actobacillus brevis KB 290 ( KB 290), isolated from a traditional J apanese pickle ‘ S uguki’, has been reported to have immunomodulatory effects. We investigated whether oral administration of KB 290 has protective effects against influenza virus ( IFV ) infection in mice. After 14 days of administration of lyophilized KB 290 suspended in phosphate‐buffered saline by oral gavage, BALB /c mice were intranasally infected with 2 × MLD 50 (50% mouse lethal dose) of IFV A/ PR /8/34 ( H 1 N 1). Prophylactically administered KB 290 significantly alleviated the loss of body weight and the deterioration in observational physical conditions induced by the infection. In addition, 7 days after infection, the levels of IFV ‐specific immunoglobulin ( I g) A in bronchoalveolar lavage fluid were significantly increased in mice fed KB 290 compared with controls. Moreover, there was a significant elevation of serum interferon ( IFN )‐α in KB 290 group mice, even at three and 7 days after infection, despite the administration of KB 290 being stopped before IFV infection. Our results demonstrated that oral administration of KB 290 before infection could alleviate IFV ‐induced clinical symptoms. Alleviation of clinical symptoms by KB 290 consumption may have been induced by long‐lasting enhancement of IFN ‐α production and the augmentation of IFV ‐specific I g A production. Significance and Impact of the Study This study demonstrated that oral administration of L actobacillus brevis KB 290 ( KB 290), a probiotic strain derived from a Japanese traditional pickle, could protect against influenza virus ( IFV ) infection in mice. Our results demonstrated that continual intake of KB 290 for 14 days prior to IFV infection alleviated clinical symptoms such as loss of body weight and deterioration in observational physical conditions induced by the infection. The beneficial effects of KB 290 consumption may have been elicited by the long‐lasting enhancement of interferon‐α production and the augmentation of IFV ‐specific immunoglobulin A production.