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Cytotoxicity and mycotoxin production of shellfish‐derived P enicillium spp., a risk for shellfish consumers
Author(s) -
Geiger M.,
Guitton Y.,
Vansteelandt M.,
Kerzaon I.,
Blanchet E.,
Robiou du Pont T.,
Frisvad J.C.,
Hess P.,
Pouchus Y.F.,
Grovel O.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12143
Subject(s) - mussel , patulin , mytilus , mycotoxin , shellfish , biology , penicillium , food science , microbiology and biotechnology , fishery , aquatic animal , fish <actinopterygii>
Abstract In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish‐farming areas, P enicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish‐derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract ( M ytilus edulis ), mussel flesh‐based medium ( MES ), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host‐derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish‐derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC ‐ UV / DAD ‐ MS / MS dereplication of extracts from P enicillium marinum and P . restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P . antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish‐derived P enicillium strains grown on a host‐derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered. Significance and Impact of the Study Penicillium strains isolated from bivalve molluscs produce extracts exhibiting a higher cytotoxicity than extracts from Penicillium strains isolated from the surrounding marine environment. The use of a mussel‐based medium for cultures of some shellfish‐derived strains enhances the cytotoxicity of extracts when compared with classical media. The production of cytotoxic compounds and of the mycotoxin patulin on such a host‐derived medium highlights a potential health risk for shellfish consumers.