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Assessment of in vitro removal of cholesterol oxidation products by L actobacillus casei ATCC 334
Author(s) -
MachorroMéndez I.A.,
HernándezMendoza A.,
Cardenia V.,
RodriguezEstrada M.T.,
Lercker G.,
Spinelli F.,
Cellini A.,
García H.S.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12132
Subject(s) - lactobacillus casei , lactic acid , bacteria , food science , biochemistry , ingestion , cholesterol , lactobacillus , chemistry , in vitro , absorption (acoustics) , microbiology and biotechnology , biology , fermentation , physics , acoustics , genetics
Cholesterol oxidation products ( COP s) are a group of compounds formed during processing and storage of foods from animal origin. After ingestion, COP s are absorbed in the intestine and can be distributed to serum and various tissues, potentially promoting a variety of toxic effects. Therefore, inhibition of their intestinal absorption may contribute to reduce the health risks associated with dietary intake of COP s. Some studies have shown that drugs and dietary compounds may inhibit the intestinal absorption of dietary COP s. However, proven cholesterol‐ and/or food toxins‐binding lactic acid bacteria have not been previously evaluated as potential COP s removal agents. The aim of this study was to assess the ability of Lactobacillus casei ATCC 334 to remove COP s in aqueous solution. Results showed the ability of both growing and resting cells to remove COP s ( ca . 30‐60%). All COP s–bacterium interactions were specific and partly reversible, being resting cells the most efficient for COP s removal in a ranking order of 7‐ KC > 7α‐OH/7β‐OH > triol > 5,6β‐EP > 5,6α‐EP > 25‐OH. Binding to the cell wall and/or cell membrane incorporation appears to be the most likely mechanisms involved on COPs removal by L. casei ATCC 334. Significance and Impact of the Study The results of this work demonstrate for the first time the ability of a specific lactic acid bacterial strain to remove cholesterol oxidation products from an aqueous solution. This finding highlights the promising biological protective role of this bacterium as a potential removal agent of these toxic compounds.