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Assignment of serotype to S almonella enterica isolates obtained from poultry and their environment in southern B razil
Author(s) -
PulidoLandínez M.,
SánchezIngunza R.,
Guard J.,
Nascimento V. Pinheiro do
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12110
Subject(s) - serotype , microbiology and biotechnology , bacteria , biology , genetics
To assess diversity of S almonella enterica serotypes present in poultry and their environment from southern B razil, the K auffmann– W hite– L e M inor ( KWL ) scheme was used to serotype a total of 155 isolates. Isolates were then re‐examined with nested PCR and sequencing of the dkg B ‐linked intergenic sequence ribotyping ( ISR ) region that assesses single nucleotide polymorphisms occurring around a 5 S ribosomal gene. Serotypes identified were H eidelberg (40·6%), E nteritidis (34·2%), H adar (8·4%), T yphimurium (3·9%), G allinarum (3·2%), A gona (1·3%), C erro (1·3%), L ivingstone (1·3%), I nfantis (0·6%), I sangi (0·6%), M bandaka (0·6%), Montevideo (0·6%) and Senftenberg (0·6%). Three unique ISR s were detected from four strains. Day old chicks yielded only S . E nteritidis, whereas S . H eidelberg was most often associated with poultry carcasses. Overall agreement between KWL and ISR was 85·2%, with disagreement possibly due to the ability of ISR to detect mixtures of serotypes in culture. Overall, ISR provided more information than did KWL about the ecology of S alm. enterica on‐farm. The O ‐antigen group D S alm . enterica serovars such as P ullorum, G allinarum and E nteritidis appear susceptible to overgrowth by other serotypes. Significance and Impact of the Study Single nucleotide polymorphisms found in a group of poultry‐associated S almonella isolates from southern Brazil provided evidence of mixtures of serovar group D serotypes on‐farm and in single samples from birds. This finding suggests that co‐infection and interserotype competition of S almonella enterica in poultry could impact the incidence of disease in animals or humans. In addition, unique serotypes were identified on‐farm that escaped characterization by antibody typing. Application of cost‐efficient and highly discriminatory genomic methods for assigning serotype may alter concepts about the epidemiology of S alm. enterica on‐farm and in foods.