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Isolation and characterization of fatty acid methyl ester ( FAME )‐producing S treptomyces sp. S 161 from sheep ( O vis aries ) faeces
Author(s) -
Lu Y.,
Wang J.,
Deng Z.,
Wu H.,
Deng Q.,
Tan H.,
Cao L.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12096
Subject(s) - fatty acid , strain (injury) , starch , fatty acid methyl ester , 16s ribosomal rna , streptomyces , biology , chemistry , biochemistry , bacteria , chromatography , biodiesel , gene , genetics , anatomy , catalysis
An actinomycete producing oil‐like mixtures was isolated and characterized. The strain was isolated from sheep faeces and identified as S treptomyces sp. S 161 based on 16 S rRNA gene sequence analysis. The strain showed cellulase and xylanase activities. The 1 H nuclear magnetic resonance ( NMR ) spectra of the mixtures showed that the mixtures were composed of fatty acid methyl esters (52·5), triglycerides (13·7) and monoglycerides (9·1) (mol.%). Based on the gas chromatography–mass spectrometry ( GC ‐ MS ) analysis, the fatty acid methyl esters were mainly composed of C 14‐ C 16 long‐chain fatty acids. The results indicated that S treptomyces sp. S 161 could produce fatty acid methyl esters ( FAME ) directly from starch. To our knowledge, this is the first isolated strain that can produce biodiesel ( FAME ) directly from starch. Significance and Impact of the Study Nowadays, production of biodiesel is based on plant oils, animal fats, algal oils and microbial oils. Lipid mostly consists of triacylglycerols ( TAG ), and conversion of these lipids into fatty acid short‐chain alcohol esters (methanol or ethanol) is the final step in biodiesel production. In this study, an oil‐producing Streptomyces strain was isolated from sheep faeces. The oil was composed of C 14 ‐C 16 long‐chain fatty acid methyl esters, triglycerides and monoglycerides. This is the first isolated strain‐producing biodiesel ( FAME ) directly from starch. Due to showing cellulase and xylanase activities, the strain would be helpful for converting renewable lignocellulose into biodiesel directly.