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Expression and subcellular localization of organophosphate hydrolase in acephate‐degrading P seudomonas sp. strain I nd01 and its use as a potential biocatalyst for elimination of organophosphate insecticides
Author(s) -
Pinjari A.B.,
Pandey J.P.,
Kamireddy S.,
Siddavattam D.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12080
Subject(s) - acephate , organophosphate , strain (injury) , microbiology and biotechnology , biology , pseudomonas , chemistry , enzyme , biochemistry , bacteria , pesticide , genetics , agronomy , anatomy
Organophosphate hydrolase ( OPH ), the product of an organophosphate‐degrading ( opd ) gene cloned from B revundimonas diminuta , hydrolyses the triester linkage found in neurotoxic organophosphate ( OP ) insecticides and nerve agents. Despite the fact that OPH s have a broad substrate range, OP compounds with a P ‐ S linkage, such as insecticides like acephate, are poor substrates for the enzyme. Expression of OPH in acephate‐utilizing P seudomonas sp. I nd01 generated a live biocatalyst capable of degrading a wide range of OP insecticides. The heterologously expressed OPH , which is a substrate of twin arginine transport ( T at) pathway, successfully targeted to the membrane of P seudomonas sp. I nd01. The membrane‐associated OPH had a size that coincided with the mature form of OPH (m OPH ), suggesting successful processing and targeting of the expressed OPH to the membrane. P seudomonas sp. Ind01 expressing OPH degraded a variety of OP insecticides besides using acephate as sole carbon source. Significance and Impact of the Study A biocatalyst capable of degrading a wide range of organophosphate ( OP ) insecticides was generated by expressing an organophosphate degradation gene in P seudomonas sp. I nd01 involved in mineralization of acephate. The biocatalyst can be used to eliminate a wide range of OP insecticide residues from the environment.