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Probiotic‐mediated competition, exclusion and displacement in biofilm formation by food‐borne pathogens
Author(s) -
Woo J.,
Ahn J.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12051
Subject(s) - probiotic , microbiology and biotechnology , lactobacillus paracasei , biofilm , lactobacillus rhamnosus , lactobacillus casei , listeria monocytogenes , lactobacillus acidophilus , biology , bacteria , food science , fermentation , genetics
Abstract The objective of this study was to examine the inhibitory effect of probiotic strains on pathogenic biofilm formation in terms of competition, exclusion and displacement. Probiotic strains ( L actobacillus acidophilus KACC 12419 , L act . casei KACC 12413, L act . paracasei KACC 12427 and L act . rhamnosus KACC 11953) and pathogens ( S almonella Typhimurium KCCM 40253 and L isteria monocytogenes KACC 12671) were used to evaluate the auto‐aggregation, hydrophobicity and biofilm formation inhibition. The highest auto‐aggregation abilities were observed in L act . rhamnosus (17·5%), L act . casei (17·2%) and L act . acidophilus (15·1%). S alm . Typhimurium had the highest affinity to xylene, showing the hydrophobicity of 53·7%. The numbers of L . monocytogenes biofilm cells during the competition, exclusion and displacement assays were effectively reduced by more than 3 log when co‐cultured with L act . paracasei and L act . rhamnosus . The results suggest that probiotic strains can be used as alternative way to effectively reduce the biofilm formation in pathogenic bacteria through competition, exclusion and displacement. Significance and Impact of the Study This study provides new insight into biofilm control strategy based on probiotic approach. Probiotic strains effectively inhibited the biofilm formation of L isteria monocytogenes through the mechanisms of competition, exclusion and displacement. These findings contribute to better understand the probiotic‐mediated competition, exclusion and displacement in biofilm formation by pathogens.