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Comparative proteomic analysis of rice seedlings in response to inoculation with B acillus cereus
Author(s) -
Wang W.,
Chen L.N.,
Wu H.,
Zang H.,
Gao S.,
Yang Y.,
Xie S.,
Gao X.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12035
Subject(s) - bacillus cereus , proteome , cereus , biology , bacillaceae , gel electrophoresis , proteomics , microbiology and biotechnology , biochemistry , bacteria , botany , genetics , gene , bacillus subtilis
Reports suggest that B acillus spp. can be used to increase plant growth and resistance to disease, but the molecular mechanisms underlying the interaction between B acillus spp. and plant is not completely understood. In the present study, to clarify these underlying mechanisms, the interaction between B acillus cereus and rice was investigated using two‐dimensional gel electrophoresis. Through comparative analysis, a total of 31 differentially expressed proteins were obtained upon B . cereus NMSL 88 treatment, including 22 proteins that were up‐regulated and nine that were down‐regulated. These data indicated that certain proteins involved in plant growth and development were up‐regulated, such as xyloglucan endotransglycosylase. Interestingly, proteins involved in defence were also up‐regulated, including peroxidases, glutathione S ‐transferases and kinases. Thus, proteins associated with disease resistance characteristics were induced in the plants after exposure to B . cereus NMSL 88. In addition, several proteins involved in protein and lipid metabolism showed significant changes in expression. Significance and Impact of the Study The present study is the first report to reveal the molecular mechanisms involved in rice seedlings in response to inoculation with B acillus cereus at the level of proteome. The results demonstrated that B . cereus NMSL 88 can up‐regulate the expression of proteins related to plant growth and defence, and lead to enhanced plant growth and disease resistance.