Premium
Genetic differentiation in P yrenophora teres f. teres populations from S yria and T unisia as assessed by AFLP markers
Author(s) -
Bouajila A.,
Zoghlami N.,
Murad S.,
Baum M.,
Ghorbel A.,
Nazari K.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12029
Subject(s) - biology , amplified fragment length polymorphism , genetic diversity , genetic variation , population , gene flow , genotyping , genotype , genetics , gene , demography , sociology
Abstract To investigate the level of genetic differentiation and diversity among P yrenophora teres isolate populations originating from different agro‐ecological areas of S yria and T unisia and to determine the potential of AFLP profiling in genotyping P yrenophora teres f . teres . In this study, AFLP markers have been employed to identify patterns of population structure in 20 P yrenophora teres f . teres populations from S yria and T unisia. Ninety‐four isolates were studied by the use of a protocol that involved stringent PCR amplification of fragments derived from digestion of genomic DNA with restriction enzymes Eco RI and M es I . Based on 401 amplified polymorphic DNA markers ( AFLP ), variance analyses indicated that most of the variation was partitioned within rather than between populations. Genotypic diversity ( GD ) was high for populations from Rihane, local landraces and different agro‐ecological zones ( GD = 0·75–0·86). There was high genetic differentiation among pathogen populations from different host populations in S yria ( G st = 0·31, ht = 0·190) and Tunisia ( G st = 0·39, ht = 0·263), which may be partly explained by the low gene flow around the areas sampled. A phenetic tree revealed three groups with high bootstrap values (55, 68, 76) and reflected the grouping of isolates based on host, or agro‐ecological areas. AFLP profiling is an effective method for typing the genetically diverse pathogen P yrenophora teres f. teres . Significance and Impact of the Study The study represents a comparative analysis of the genetic diversity in P . teres isolates from two countries spanning two continents and also shows that several distinct P . teres genotypes may be found in a given environment. The implications of these findings for P yrenophora teres f. teres evolutionary potential and net blotch‐resistance breeding in S yria and T unisia were also discussed.