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Lead‐resistant P rovidencia alcalifaciens strain 2 EA bioprecipitates Pb +2 as lead phosphate
Author(s) -
Naik M.M.,
Khanolkar D.,
Dubey S.K.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12026
Subject(s) - bioremediation , providencia , chemistry , nuclear chemistry , scanning electron microscope , phosphate , environmental chemistry , bacteria , materials science , biochemistry , biology , enterobacter , genetics , escherichia coli , gene , composite material
Abstract A lead‐resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l −1 by precipitating soluble lead as insoluble light brown solid. Scanning electron microscopy coupled with energy‐dispersive X‐ray spectrometric analysis (SEM‐EDX) and X‐ray diffraction spectroscopy (XRD) revealed extracellular light brown precipitate as lead orthophosphate mineral, that is, Pb 9 (PO 4 ) 6 catalysed by phosphatase enzyme. This lead‐resistant bacterial strain also demonstrated tolerance to high levels of cadmium and mercury along with multiple antibiotic resistance. Providencia alcalifaciens strain 2EA could be used for bioremediation of lead‐contaminated environmental sites, as it can efficiently precipitate lead as lead phosphate. Significance and Impact of the Study Providencia alcalifaciens strain 2EA resist lead nitrate up to 0·0014 mol l −1 by precipitating soluble lead (Pb +2 ) as insoluble light brown solid. Scanning electron microscopy coupled with energy‐dispersive X‐ray spectrometric analysis (SEM‐EDX) and X‐ray diffraction spectroscopy (XRD) revealed extracellular light brown precipitate as lead orthophosphate mineral, that is, Pb 9 (PO 4 ) 6 catalysed by phosphatase enzyme. Providencia alcalifaciens strain 2EA could be used for bioremediation of lead‐contaminated environmental sites, as it can efficiently precipitate lead as insoluble lead phosphate.