Premium
Evaluation of the host specificity of B acteroides thetaiotaomicron alpha‐1‐6, mannanase gene as a sewage marker
Author(s) -
Aslan A.,
Rose J.B.
Publication year - 2013
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12013
Subject(s) - bacteroides thetaiotaomicron , bacteroides , biology , bacteroidaceae , sewage , feces , genetic marker , molecular marker , marker gene , microbiology and biotechnology , polymerase chain reaction , gene , bacteria , genetics , engineering , waste management
The specificity of a short fragment functional gene marker (alpha‐1‐6, mannanase) in B acteroides thetaiotaomicron was compared with two other B acteroidales markers used for identifying sewage as the source of pollution in water. A total of 11 different animal species and 230 faecal samples were tested using quantitative polymerase chain reaction. The results showed that the alpha‐1‐6, mannanase marker had high specificity (0·97). Limited cross‐reactivity was found in swine, gull and cat faeces with copy numbers close to detection limit. Positives were detected in all bovine and sheep faeces tested for the Human‐Bac1 marker. The human‐specific alpha‐1‐6, mannanase marker showed greater specificity (0·97) than the HF 183 B acteroides PCR marker (0·71) and the H uman‐ B ac1 ( qPCR ) marker. Significance and Impact of the Study This is the first study that compares Bacteroides thetaiotaomicron alpha‐1‐6, mannanase marker with other markers. Our study showed that this marker has strong potential to be used as an indicator for sewage pollution in water.