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Enhancement of ethanol production from potato‐processing wastewater by engineering E scherichia coli using V itreoscilla haemoglobin
Author(s) -
Abanoz K.,
Stark B.C.,
Akbas M.Y.
Publication year - 2012
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/lam.12000
Subject(s) - aeration , ethanol fuel , food science , hydrolysate , escherichia coli , wastewater , ethanol , biomass (ecology) , biofuel , strain (injury) , volume (thermodynamics) , biology , chemistry , microbiology and biotechnology , biochemistry , agronomy , environmental science , environmental engineering , gene , ecology , hydrolysis , physics , anatomy , quantum mechanics
Ethanologenic E scherichia coli strain FBR 5 was transformed with the V itreoscilla haemoglobin ( VHb ) gene ( vgb ) in two constructs (resulting in strains TS 3 and TS 4). Strains FBR 5, TS 3 and TS 4 were grown at two scales in LB medium supplemented with potato‐processing wastewater hydrolysate. Aeration was varied by changes in the medium volume to flask volume ratio. Parameters measured included culture pH , cell growth, VH b levels and ethanol production. VH b expression in strains TS 3 and TS 4 was consistently correlated with increases in ethanol production (5–18%) under conditions of low aeration, but rarely did this occur with normal aeration. The increase in ethanol yields under low aeration conditions was the result of enhancement of ethanol produced per unit of biomass rather than enhancement of growth. ‘ VH b technology’ may be a useful adjunct in the production of biofuels from food‐processing wastewater. Significance and Impact of Study Genetic engineering using Vitreoscilla haemoglobin (VHb) has been shown previously to increase ethanol production by Escherichia coli from fermentation of the sugars in corn fibre hydrolysate. The study reported here demonstrates a similar VHb enhancement of ethanolproduction by fermentation of the glucose from potato waste water hydrolysate and thus extends the list of sugar containing waste products from which ethanol production may be enhanced by this strategy.