Open AccessNew evidence of cryptic speciation in the family Longidoridae (Nematoda: Dorylaimida)
Author(s) -
Cai Ruihang,
ArchidonaYuste Antonio,
CantalapiedraNavarrete Carolina,
PalomaresRius Juan E.,
Castillo Pablo
Publication year - 2020
Publication title -
journal of zoological systematics and evolutionary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.769
H-Index - 50
eISSN - 1439-0469
pISSN - 0947-5745
DOI - 10.1111/jzs.12393
Subject(s) - biology , xiphinema , species complex , taxonomy (biology) , morphometrics , genetic algorithm , dorylaimida , zoology , phylogenetic tree , evolutionary biology , ecology , nematology , genetics , gene , nematode
Longidorid nematodes comprise more than 500 species, and Longidorus and Xiphinema are the most diversified, prevalent, and cosmopolitan genera within plant‐parasitic nematodes. This increases the risk of species misidentification. We conducted an integrative morphometric and genetic study on two longidorid species to elucidate the existence of new cases of cryptic speciation within the genera Longidorus and Xiphinema. Detailed morphological, morphometrical, multivariate, and genetic studies were carried out, and mitochondrial and nuclear haploweb analyses were used to differentiate species within the L. iliturgiensis and X. hispanum complexes. Species delimitation using multivariate and haplonet tools of L. iliturgiensis species complex clearly separated L. tabernensis sp. nov. from L. iliturgiensis and L. indalus , and X. subbaetense sp. nov. from X. hispanum and X. adenohystherum . D2‐D3 , partial 18S , and partial coxI regions were used for inferring their phylogenetic relationships with other species in each genus. The present study provides new insights into the diversity of Longidorus and Xiphinema species detected in southern Spain, and new evidence of cryptic speciation in both genera. These results support our hypothesis that the biodiversity of Longidoridae in southern Europe is higher than previously supposed and is still not fully clarified.