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Quantitative estimation of islet tissue of pancreas in possums (Trichosurus vulpecula)
Author(s) -
Edwin Nalini
Publication year - 1982
Publication title -
journal of zoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.915
H-Index - 96
eISSN - 1469-7998
pISSN - 0952-8369
DOI - 10.1111/jzo.1982.197.4.455
Subject(s) - pancreas , biology , islet , anatomy , head and neck , endocrinology , diabetes mellitus , medicine , surgery
The pancreas of six possums, both male andi female were isolated. The relationship between the weight of the pancreas and the weight of the animal was then calculated. Sections were stained with haemotoxylin and eosin and islet tissue mass estimated using a linear scanning technique in each of the regions of the pancreas: head neck, body and tail. A uniform distribution was shown. The relationship between the average islet mass and body weight was calculated. Sections were stained with aldehyde fuchsin in each of the regions, head, neck, body and tail using a Wild‐M501 semiautomatic sampling microscope. Beta Grimelius' silver nitrate stain in each of the regions: head, neck, body and tail. The alpha cells were also estimated by using a Wild‐M501 sampling microscope and a uniform distribution was shown.