Open AccessExpression of insulin‐like growth factor genes in olive flounder, Paralichthys olivaceus , fed a diet with partial replacement of dietary fish meal
Author(s) -
Moon JiSung,
Oh Dae Han,
Park SuJin,
Seo JinSong,
Kim DongUk,
Moon SeongHwan,
Park Hung Sik,
Lim SangGu,
Lee BongJoo,
Hur SangWoo,
Lee KyeongJun,
Nam Taek Jeong,
Choi Youn Hee
Publication year - 2023
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12914
Subject(s) - olive flounder , paralichthys , biology , fish meal , medicine , endocrinology , meal , insulin like growth factor , growth hormone , gene expression , soybean meal , growth factor , hormone , gene , food science , fishery , fish <actinopterygii> , biochemistry , ecology , raw material , receptor
Abstract This study analyzed the expression of growth‐related genes of olive flounder, Paralichthys olivaceus , fed a low‐fish meal (FM) diet to investigate the replacement of the FM diet in the field. The expression of growth hormone (GH)/insulin‐like growth factor (IGF) axis genes in the brain/pituitary/liver and plasma hormone concentrations were measured. A basal experimental diet was formulated using 63% FM and 12% soybean meal as the primary protein sources, and then two other diets were made by replacing 20% and 30% of the FM with soy protein concentrate, tankage meal, and poultry by‐products meal. Each diet was fed to duplicate groups of juvenile flounder (150 ± 3.0 g) twice a day. After 20 weeks, the plasma growth‐related hormone concentrations were similar between the control and FM20 groups. Moreover, GH/IGF axis gene mRNA expression in the brain/pituitary/liver was similar between the control and FM20 groups. Immunoblotting of muscle and liver showed the same pattern. This study showed the utility of replacing 20% of the FM diet and supports the possibility of field application.