Open AccessEffect of time in culture on fatty acid composition of copepods Tisbe sp . and Apocyclops sp .
Author(s) -
Alejos Cabrera Ruth M.,
Gaspar Reyes Wilmer A.,
Flores Ramos Leenin,
Ynga Huamán Gheraldine A.,
Ruiz Soto Anthony,
Niño Velásquez Alexander F.
Publication year - 2022
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12827
Subject(s) - biology , docosahexaenoic acid , polyunsaturated fatty acid , arachidonic acid , eicosapentaenoic acid , fatty acid , food science , composition (language) , isochrysis galbana , zoology , biochemistry , botany , larva , linguistics , philosophy , enzyme
The purpose of this study was to evaluate the effect of time in culture on fatty acid composition of copepods Tisbe sp . and Apocyclops sp . For experiments, Tisbe and Apocyclops copepodites were separated and placed in 4‐L containers at 5 ind/mL. The fatty acids were evaluated at three different periods (hours) of culture (treatments): T1 = 6 hr, T2 = 12 hr, and T3 = 168 hr. Culture conditions were 25°C, salinity 35 ppt, illuminance 2,500 lx, with gentle aeration. The live food was Isochrysis galbana at 75 × 10 3 cells/mL. A total of 14 fatty acids were detected for both copepods. Although, there were absent two fatty acids for Apocyclops sp . (C18:3n‐6 and C18:1n‐7). The major percentages of fatty acids were more than 50% of polyunsaturated fatty acids. The essential fatty acids were detected such as C22:6n‐3 (docosahexaenoic acid [DHA]), C20:5n‐3 (eicosapentaenoic acid [EPA]), and C20:4n‐6 (arachidonic acid) for both species. Tisbe sp . was the species which shows the highest quantity of total lipids compared to Apocyclops sp . for the three treatments. Besides, in T3 both species had greater quantities of total lipids in respect to the first treatment. The ratio of DHA/EPA was optimal for culture of fish marine larvae because the proportion is higher than 2:1.