Open AccessDietary supplementation of host‐associated lactic acid bacteria modulates growth, metabolic activities, and immune‐related gene expression in giant freshwater prawn, Macrobrachium rosenbergii
Author(s) -
Kader Md. Abdul,
Zahidah Azahar Nursyafiqah,
Iehata Shumpei,
Bulbul Mahbuba,
Islam Md. Moudud,
Sarker Joyshri,
Mariom ,
Rahman Md. Moshiur,
Asaduzzaman Md
Publication year - 2021
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12734
Subject(s) - biology , macrobrachium rosenbergii , lactococcus lactis , lactic acid , feed conversion ratio , probiotic , transaminase , hepatopancreas , immune system , zoology , bacteria , prawn , food science , microbiology and biotechnology , biochemistry , endocrinology , enzyme , immunology , fishery , body weight , genetics
Abstract The present study was carried out to evaluate the dietary effects of host‐associated lactic acid bacteria on growth performance, metabolic enzyme activities, and immune response of Macrobrachium rosenbergii juveniles. To formulate the test diets, a control (Con) diet was supplemented with a commercial probiotic and three host‐derived bacteria Enterococcus faecalis (EC), Lactococcus lactis I (LC‐I), and L. lactis II (LC‐II), which were previously isolated from the gastrointestinal tract of adult individuals of M. rosenbergii . Juvenile M. rosenbergii (0.65 ± 0.008 g) were randomly stocked at 20 individuals/100 L of fiberglass tanks with three replications for each test diet. After 50 days, juveniles fed the diets LC‐I and LC‐II showed significantly higher ( p < .05) weight gain, specific growth rate, and the lowest feed conversion ratio. The analyses of glutamic oxaloacetate transaminase and glutamic pyruvate transaminase in muscle and hepatopancreas revealed significantly ( p < .05) reduced values in LC‐I fed juveniles. The total hemocyte count and phenoloxidase activity were significantly increased ( p < .05) in LC‐I and LC‐II fed juveniles. The relative mRNA expression patterns of immune‐related α2‐M, LGBP, ProPO, Cu, Zn‐SOD, TG, PE, AKP, and ACP genes were significantly ( p < .05) upregulated in juveniles fed with LC‐I followed by the diet LC‐II. Finally, the study suggests that the growth performance and immune response of M. rosenbergii can be improved through supplementation of host‐associated L. lactis bacteria for its higher production.