Open AccessResponses of some innate immune‐genes involved in the toll‐pathway in black tiger shrimp ( Penaeus monodon ) to Vibrio harveyi infection and on exposure to ligands in vitro
Author(s) -
Deepika Anand,
Sreedharan Krishnan,
Rajendran Kooloth Valappil
Publication year - 2020
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12723
Subject(s) - penaeus monodon , biology , innate immune system , shrimp , microbiology and biotechnology , toll like receptor , immune system , vibrio harveyi , white spot syndrome , vibrio alginolyticus , pattern recognition receptor , immunology , vibrio , genetics , bacteria , fishery
In shrimp, which rely largely on their innate immune system for defense against invading pathogens, the Toll‐pathway has been extensively studied, as it plays a crucial role in innate immune responses. Different innate immune genes in the Toll‐pathway of shrimp, Penaeus monodon viz., Toll‐Like receptor ( Pm Toll), Myeloid differentiation factor‐88 ( Pm MyD88), and Tumor necrosis factor receptor associated factor‐6 ( Pm TRAF6) have been characterized and their role in immune response against white spot syndrome virus has been elucidated by real‐time PCR‐based expression analysis. In the current study, the role of these genes in the immune response against bacteria was investigated by determining the mRNA expressions of Pm Toll, Pm MyD88, and Pm TRAF6 in different tissues of P. monodon , upon experimental infection with Vibrio harveyi , using real‐time PCR. Seven tissues were selected, including hemocytes, lymphoid organ, gill, hepatopancreas, stomach, midgut, and hindgut. Significant upregulation of these genes was observed in hemocytes, lymphoid organ, and gut tissues at different time‐points after infection. Moreover, modulation of expression of the genes at different time‐points could be observed in primary cultured hemocytes in vitro upon exposure with pathogen‐mimicking ligands (lipopolysacchrides and peptidoglycans). These experimental results indicate that the Toll‐pathway plays an important role in the immune response against bacterial challenge.