Open AccessDifferential expression of akirin gene in black tiger shrimp Penaeus monodon in response to immunostimulant administration and infections with Vibrio harveyi and white spot syndrome virus
Author(s) -
Dechamma Mundanda M.,
Mani Madhu K.,
Rajeish Moger,
Santhosh Kogaluru S.,
Akhila Dharnappa S.,
Maiti Biswajit,
Bossier Peter,
Karunasagar Iddya,
Karunasagar Indrani
Publication year - 2020
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12704
Subject(s) - white spot syndrome , penaeus monodon , biology , shrimp , vibrio harveyi , hepatopancreas , prophenoloxidase , gene expression , microbiology and biotechnology , immunostimulant , gene , virology , innate immune system , immunology , immune system , vibrio , fishery , genetics , biochemistry , bacteria
The akirin gene, which is strictly localized in the nucleus, plays a critical role in regulating antimicrobial peptide transcription, and has parallel functions to NF‐κB signaling pathway in both vertebrates and invertebrates. In shrimp, the akirin gene is expressed as innate immunity in response to microbial infection. In the present study, expression of akirin gene in Penaeus monodon with respect to Vibrio harveyi and white spot syndrome virus (WSSV) infections and immunostimulant (β‐glucan) administration were investigated by quantitative polymerase chain reaction. The gene was expressed in various tissue samples of healthy shrimp. Maximum level of expression was immediately after V. harveyi infection, suggesting that it may be an early response gene. Gene expression was remarkably upregulated in the lymphoid organ, gill, and hepatopancreas, whereas downregulation was observed in hemocytes compared with the control. In the case of WSSV‐infected samples, the akirin gene was significantly downregulated in the lymphoid organ but there was no significant difference in expression pattern in hemocytes compared to the control. In gill tissue, maximum expression was observed after 2 hr of infection, the same in hepatopancreas. Experimental challenge of β‐glucan fed shrimp infected with V. harveyi and WSSV resulted in significant upregulation of akirin gene expression in lymphoid and gill tissue.