Open AccessDiscriminant analysis of four cultured populations of the pearl oyster, Pinctada fucata martensii , using morphology and single‐nucleotide polymorphism markers
Author(s) -
Huang Jing,
Xu Meng,
He Maoxian
Publication year - 2020
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12678
Subject(s) - biology , single nucleotide polymorphism , genetics , population , genetic diversity , germplasm , allele , pinctada fucata , genotype , gene , pearl , botany , pearl oyster , philosophy , demography , theology , sociology
In this study, genetic diversity and genetic structure were assessed in the four major cultured populations of P. fucata martensii from Shenzhen (SZ), Hainan (HN), Zhanjiang (ZJ), and hybrid (HY), based on morphological multivariate analysis and 25 single‐nucleotide polymorphism (SNP) markers. The morphological characteristics of HN and HY populations were similar, and the average discriminant accuracy was 75.6%. All SNP loci comprised two alleles, and the average observed ( H o ) and expected ( H e ) heterozygosity of HY, SZ, HN, and ZJ populations were 0.2236–0.2734 and 0.2374–0.2870, respectively. The average polymorphism information content values of HY, SZ, HN, and ZJ populations were 0.1923–0.2272. Gene flow between HY and HN populations was frequent, with an N m value of 6.0928, while that between SZ and the other three populations was less frequent. The combined cluster, genetic distance, and gene flow analyses indicate that the genetic background of HY and HN populations is similar, and they can be regarded as a single population. The ZJ population might originate from the HY population, while the SZ population should be independent. These results could assist the management of germplasm resources and selective breeding of pearl oysters.