Open AccessDietary probiotic Pediococcus acidilactici MA18/5M modulates the intestinal microbiota and stimulates intestinal immunity in rainbow trout ( Oncorhynchus mykiss )
Author(s) -
AlHisnawi Ali,
Rodiles Ana,
Rawling Mark D.,
Castex Mathieu,
Waines Paul,
Gioacchini Georgia,
Carnevali Oliana,
Merrifield Daniel L.
Publication year - 2019
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12642
Subject(s) - pediococcus acidilactici , rainbow trout , biology , probiotic , microbiology and biotechnology , trout , brown trout , fishery , fish <actinopterygii> , bacteria , lactic acid , lactobacillus plantarum , genetics
A study was conducted to evaluate the probiotic effect of Pediococcus acidilactici MA18/5M on rainbow trout, Oncorhynchus mykiss . Fish (310 ± 9 g) were fed a control diet or a P. acidilactici ‐supplemented diet (at 2.4 × 10 6 CFU/g) for 4 weeks. The probiotic was observed to populate the intestine with levels ranging from log 3.7 to 5.4 CFU/g. Furthermore, these populations were able to persist for at least 24 hr after the cessation of probiotic feeding. High‐throughput sequencing analysis of bacterial 16S rRNA libraries demonstrated that P. acidilactici was able to modulate the gut microbiome of rainbow trout and that the probiotic was detected as a common taxon on the mucosa and in the digesta of the probiotic fish ( p < .05). Real‐time polymerase chain reaction demonstrated that feeding the probiotic upregulated pro‐inflammatory cytokines, interleukin‐1β, and interleukin‐8 and downregulated anti‐inflammatory interleukin‐10 compared to the control‐fed fish. Furthermore, the mRNA levels for the mucosal antibody immunoglobulin T was also elevated in probiotic‐fed fish. These findings help to explain some of the mechanisms behind the previously reported observed benefits of using this probiotic in the intestinal morphology and immunity of rainbow trout.