Open AccessEnhanced muscle regeneration in freshwater prawn Macrobrachium rosenbergii achieved through in vivo silencing of the myostatin gene
Author(s) -
Easwvaran Sarasvathi P.,
Bhassu Subha,
Maningas Mary B. B.,
Othman Rofina Y.
Publication year - 2019
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12607
Subject(s) - biology , myostatin , macrobrachium rosenbergii , gene silencing , tropomyosin , myosin , gene , microbiology and biotechnology , gene expression , regeneration (biology) , prawn , genetics , fishery
Myostatin (MSTN) is an interesting negative growth‐regulating gene that has been well characterized in vertebrates but scantly described in invertebrates. The current study focuses on the downregulation of the Mr MSTN gene and subsequently records any histological changes for giant freshwater prawn, Macrobrachium rosenbergii ( Mr ). In addition, the study also deals with the Mr MSTN gene's influence on other growth‐related genes, which include myosin heavy chain, dystrophin‐dystroglycoprotein complex, tropomyosin, farnesoic acid o‐methyl transferase, arginine kinase, cyclophilin, and acyl CoA desaturase. The preliminary histological analysis following Mr MSTN silencing favors muscle regeneration, which supports its functional role as a negative growth regulator and its significant effect on the expression of other growth‐related genes. Overall, our results show that the Mr MSTN gene could therefore be a potential target for gene manipulation aimed at enhancing the growth and muscle development of M. rosenbergii, which could be beneficial in increasing the total mass production in the postlarva phase at the hatchery level.