Open AccessMolecular Cloning, Characterization, and Expression of Crustacean Cardioactive Peptide in Oriental River Prawn, Macrobrachium nipponense , during Acute Hypoxia and Reoxygenation
Author(s) -
Shan Dongyan,
Fu Hongtuo,
Qiao Hui,
Sun Shengming,
Zhang Wenyi,
Jin Shubo,
Jiang Sufei,
Gong Yongsheng,
Xiong Yiwei,
Wu Yan
Publication year - 2018
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12502
Subject(s) - eyestalk , biology , hypoxia (environmental) , prawn , crustacean , macrobrachium , cloning (programming) , decapoda , microbiology and biotechnology , biochemistry , fishery , zoology , oxygen , chemistry , organic chemistry , programming language , computer science
A full‐length complementary DNA of the crustacean cardioactive peptide (CCAP) was cloned from the eyestalk of the oriental river prawn, Macrobrachium nipponense , and named Mn ‐CCAP. Experimental treatment was set to decrease dissolved oxygen levels and generate hypoxic culture conditions. The Mn ‐CCAP expression profile was different in the brain and eyestalk. Results suggested Mn ‐CCAP responds strongly to hypoxia‐induced oxidative stress. Different expression profiles in the brain and eyestalk indicate that these two tissues may respond in different ways to hypoxia.