
Pharmacokinetics of Eugenol in Japanese Flounder, Paralichthys olivaceus
Author(s) -
Tago Akio,
Yokoyama Saichiro,
Ishikawa Manabu,
Koshio Shunsuke
Publication year - 2018
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12438
Subject(s) - eugenol , paralichthys , olive flounder , flounder , pharmacokinetics , plasma concentration , intramuscular injection , chemistry , pharmacology , biology , zoology , fish <actinopterygii> , fishery , medicine , organic chemistry
Eugenol, which has been used as an anesthetic for fish, was administered to Japanese flounder, Paralichthys olivaceus (127 ± 50.8 g), by bath treatment at concentrations of 0.025, 0.125, and 0.25 mL / L seawater for 10 min and intramuscular injection at 40 μL/fish to investigate the pharmacokinetic characteristics of eugenol in the plasma of Japanese flounder. In the bath treatment test, plasma eugenol concentration increased with increasing eugenol concentration up to 0.125 mL /L and reached steady state within 5 min. After a 10‐min bath treatment in 0.25 mL /L eugenol, plasma eugenol concentration was about 58.4 µg/ mL . After transfer into running seawater, plasma eugenol concentration decreased biphasically with half‐lives of 0.0296 h (α‐phase) and 0.289 h (β‐phase). The AUC 0000 →0800 was about 16.5 µg h/ mL . In administration by intramuscular injection, plasma eugenol concentration increased rapidly after administration and decreased biphasically with half‐lives of 0.0329 h (α‐phase) and 8.08 h (β‐phase). The AUC 0000 →0800 was about 52.5 µg h/ mL . In both methods of administration, Japanese flounder with average weight of 127 g were effectively anesthetized when plasma eugenol concentrations were between 2.19 and 4.88 µg/ mL .