Open AccessPrediction of Genetic Variation in Stocks of Offspring and Its Correlation with Viability and Growth Rate
Author(s) -
Kaczmarczyk Dariusz,
Dobosz Stefan,
Kaczor Aleksandra
Publication year - 2017
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12396
Subject(s) - biology , offspring , genetic variation , loss of heterozygosity , rainbow trout , microsatellite , genetic variability , genetics , zoology , evolutionary biology , allele , fishery , genotype , fish <actinopterygii> , gene , pregnancy
This study tested the accuracy of predictions of genetic variation with Genassemblage 1.0 software and investigated whether offspring with greater genetic variation have higher survivability in aquaculture conditions than offspring with less genetic variation. To achieve these aims, microsatellite polymorphism was used to determine genetic variation in rainbow trout, Oncorhynchus mykiss , spawners and progeny. Spawning pairs were assembled based on the estimated genetic variation of their offspring. The offspring were divided into two groups: (H) group had higher expected values of heterozygosity (0.93) and allelic richness (41), whereas (L) group had lower expected values (0.63 and 31). The heterozygosity, survivability, and growth rate of these groups were recorded. The observed heterozygosity was 0.93 in (H) group and 0.62 in (L) group. (H) group had slightly higher survivability (89.8%) than (L) group (87.6%), but this difference was not significant ( p ≥ 0.05), possibly due to residual tetrasomy in the rainbow trout genome. In the offspring, predicted and observed variations were very similar, indicating that Genassemblage 1.0 accurately predicts genetic variation in progeny.