Open AccessEffects of Dietary Lysine Levels on Growth, Intestinal Digestive Enzymes, and Coelomic Fluid Nonspecific Immune Enzymes of Sea Cucumber, Apostichopus japonicus , Juveniles
Author(s) -
Liu Chenmin,
Han Yuzhe,
Ren Tongjun,
Jiang Zhiqiang,
Wang Fuqiang,
Liao Mingling,
Wang Jing
Publication year - 2017
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12344
Subject(s) - apostichopus japonicus , biology , lysine , sea cucumber , alkaline phosphatase , weight gain , food science , acid phosphatase , enzyme , digestive enzyme , protease , dry matter , zoology , biochemistry , amino acid , amylase , body weight , endocrinology , ecology
A 56‐d feeding trial was conducted to evaluate the effects of dietary lysine levels on growth performance, intestinal digestive enzymes, and nonspecific immune response of Apostichopus japonicus juveniles. Five semipurified diets were formulated to contain graded levels of lysine (from 0.28 to 2.32% of dry matter). Although no significant differences ( P > 0.05) were observed in survival rate, final weight ( FW ), and body weight gain ( BWG ), the FW and BWG increased from 0.28 to 1.19% with increasing dietary lysine levels, then showed a declining tendency. Broken‐line regression analysis of BWG indicated sea cucumber requires lysine at 0.76% of dry diet. Proximate composition, lysine content of the body wall, and total amino acid profiles were not affected by dietary treatments. Intestinal protease level increased with increasing dietary lysine level from 0.28 to 1.19%, then decreased. Acid phosphatase and superoxide dismutase activities of the sea cucumbers were not affected by various dietary lysine levels; however, significantly ( P < 0.05) higher alkaline phosphatase activity was observed in sea cucumbers fed 2.32% lysine diet than those fed diets having 0.28 and 1.89% lysine. Overall, sea cucumber juveniles fed with diets containing between 0.76 and 1.19% lysine showed better growth performance, digestive enzymes, and nonspecific immune enzyme activities.