Open AccessUse of Microsatellite DNA Profiling to Identify Japanese Flounder, Paralichthys olivaceus of Hatchery Origin
Author(s) -
Liu Yongxin,
Yang Runqing,
Liu Yi,
Si Fei
Publication year - 2017
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12330
Subject(s) - biology , paralichthys , microsatellite , broodstock , olive flounder , hatchery , sire , genetic diversity , fishery , zoology , population , genetic marker , genetics , aquaculture , fish <actinopterygii> , gene , demography , allele , sociology
Abstract Japanese flounder, Paralichthys olivaceus , is an economically important marine fish species in Asia. A suite of 18 microsatellite markers chosen from published genetic linkage maps was used to carry out parentage assignments of 188 hatchery‐reared juveniles from a small number of breeders. The probabilities of exclusion for the 18 microsatellite markers were 0.604–0.913, and the effectiveness of combined probability of exclusion reached 100% when using the eight microsatellite markers with higher Excl 1 probabilities. The cultured and wild stocks (WSs) were differentiated in a release‐recapture population based on these markers. Of the 321 recaptured offspring, 28.34% were assigned to their parental pairs in our broodstock, whereas the remaining offspring could not be traced back to a possible sire or dam. Significant reduction in genetic diversity of the cultured stock (CS) had not been found compared with that of the WS. The results suggest that CSs released into the wild will not adversely affect the genetic structure of natural populations. Our results demonstrate that these markers provide an efficient tool for parentage assignments and genetic analysis of Japanese flounder.