Open AccessTargeted Sequencing of Myogenic Regulatory Factors and Myostatins Reveals an Association between MSTN ‐1 and Interorbital Distance in Orange‐spotted Grouper, Epinephelus coioides (Hamilton, 1822)
Author(s) -
Guo Liang,
Xia Junhong,
Li Mingming,
Yang Sen,
Lin Haoran,
Meng Zining
Publication year - 2016
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12303
Subject(s) - biology , genetics , grouper , myf5 , epinephelus , population , single nucleotide polymorphism , linkage disequilibrium , myogenin , gene , evolutionary biology , fishery , genotype , transcription factor , fish <actinopterygii> , demography , sociology
Abstract The aim of this study was to investigate single nucleotide polymorphisms ( SNPs ) in genes that are presumed to control muscle growth and to determine their potential association with growth in a cultured population of orange‐spotted grouper, Epinephelus coioides . Seven genes, myogenic regulatory factor 4 ( MRF4 ), Myf5, MSTN ‐1, MSTN ‐2, MyoD1 , MyoD2 , and myogenin, were selected for the investigation, covering approximately 26 kbp. First, the two clades for the genes MSTN (myostatin) and MyoD were confirmed in this species using Bayesian inference analysis of the phylogenetic relationships. Then, the seven genes were enriched by polymerase chain reaction and sequenced using an Ion Torrent Personal Genome Machine. A total of 586 SNPs were discovered. Linkage disequilibrium was decayed by 50% within 250 bp based on the combined data, which means that there was high resolution in the association mapping. A mixed linear model considering the population structure and kinship was used to detect the associations between genotypes and phenotypes. Only one site ( KR269814 .1:g. 22T >G) in MSTN ‐1 was found to be significantly associated with a measured trait, the interorbital distance (false discovery ratio < 0.05), and it explained 12.4% of the phenotype variation of this trait. This study provides insight on strategies for molecular marker‐assisted breeding in orange‐spotted grouper.