Open AccessImmunopotentiating Effect of Small Peptides on Primary Culture Coelomocytes of Sea Cucumber, Apostichopus japonicus
Author(s) -
Wang Shuxian,
Li Tianbao,
Xu La,
Li Le,
Fan Ying,
Wei Jianteng
Publication year - 2015
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12197
Subject(s) - apostichopus japonicus , sea cucumber , biology , respiratory burst , immunostimulant , acid phosphatase , alkaline phosphatase , superoxide dismutase , immune system , phagocytosis , peptide , incubation , biochemistry , microbiology and biotechnology , enzyme , immunology , ecology
Abstract The in vitro effect of small peptides on the nonspecific immune response of sea cucumber, Apostichopus japonicus , coelomocytes was studied. Coelomocytes were cultured in L‐15 medium containing different concentrations of small peptide (0, 5, 25, and 100 µg/ mL ). Coelomocytes were incubated with the above immunostimulant for 1, 3, 6, 12, and 24 h. Coelomocytes incubated in the medium without immunostimulants were taken as controls at each incubation time. After exposure to small peptides, the coelomocytes were sampled and immune parameters namely, phagocytosis activity, respiratory burst activity, as well as activities of acid phosphatase ( ACP ), alkaline phosphatase ( AKP ), and superoxide dismutase ( SOD ) were determined. All immune parameters were significantly ( P < 0.05) enhanced by small peptides. Both phagocytic capacity and respiratory burst activity were up to maximum values at 6 h, and these values were 1.6 and 1.1 times as high as the control group, respectively. Among the humoral responses, the activities of ACP , AKP , and SOD were also up to maximum values at 6 h. The values were 1.4, 1.3, and 1.4 times as high as the control group, respectively. These results suggest that small peptides can enhance the nonspecific immune response of sea cucumber in vitro .