Research Library

open-access-imgOpen AccessEffect of Bacteriophages on Vibrio alginolyticus Infection in the Sea Cucumber, Apostichopus japonicus (Selenka)
Author(s)
Zhang Jiancheng,
Cao Zhenhui,
Li Zhen,
Wang Lili,
Li Huaqiang,
Wu Feifei,
Jin Liji,
Li Xiaoyu,
Li Shuying,
Xu Yongping
Publication year2015
Publication title
journal of the world aquaculture society
Resource typeJournals
PublisherWiley Subscription Services
Abstract Vibrio alginolyticus is an important pathogen that causes a variety of diseases in marine animals including the sea cucumber, Apostichopus japonicus. Herein, we describe a two‐phage mixture which may have potential for use as an antibacterial agent to prevent V. alginolyticus infection in the sea cucumber. A Z1210 bacterial isolate was cultured from diseased sea cucumber suffering from skin ulcerations and viscera ejection. The isolate was identified as V. alginolyticus by morphology and sequence similarity analysis. Subsequently, two bacteriophages infecting isolate Z1210 were isolated from the drainpipe of an aquatic market. Morphologically, these two phages were classified as members of Podoviridae ( PVA1 ) and Myoviridae ( PVA2 ) and each phage showed high virulence in an in vitro experiment. Additionally, an experiment conducted in a marine environment showed that a mixture of the two phages increased the survival of sea cucumbers (10 ± 2 g) to 73, 50, and 47% when it was used with a multiplicity of infection of 10, 1, or 0.1, respectively. This result differed markedly from the control without phage (3% survival) while there was no significant difference between the 80 and 47% survival observed for two antibiotic treatments (5 mg/L doxycycline and 10 mg/L kanamycin, respectively).
Subject(s)apostichopus japonicus , bacteria , biology , ecology , genetics , microbiology and biotechnology , sea cucumber , vibrio , vibrio alginolyticus , zoology
Language(s)English
SCImago Journal Rank0.655
H-Index60
eISSN1749-7345
pISSN0893-8849
DOI10.1111/jwas.12177

Seeing content that should not be on Zendy? Contact us.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here