Open AccessEffect of Dietary N ‐acyl Homoserin Lactonase on the Immune Response and the Gut Microbiota of Zebrafish, Danio rerio , Infected with Aeromonas hydrophila
Author(s) -
Cao Yanan,
Liu Yuchun,
Mao Wei,
Chen Ruidong,
He Suxu,
Gao Xiuhua,
Zhou Zhigang,
Yao Bin
Publication year - 2014
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12105
Subject(s) - aeromonas hydrophila , biology , quorum sensing , danio , microbiology and biotechnology , zebrafish , immune system , gut flora , chromobacterium violaceum , bacteria , virulence , biochemistry , immunology , gene , genetics
Quorum sensing interference is a novel strategy in bacterial disease control. Degradation of quorum sensing signal molecules, such as N ‐acyl homoserine lactones ( AHLs ), by enzymes is a potential interfering method. In this study, the in vivo effect of an N ‐acyl homoserine lactonase from Bacillus sp. B546 , AiiA B546 , on the protection of zebrafish, Danio rerio , against Aeromonas hydrophila infection was first investigated by co‐feeding. The short‐term immune responses and the long‐term effects on the gut microbiota of zebrafish were analyzed by quantitative polymerase chain reaction ( qPCR ) and denaturing gradient gel electrophoresis ( DGGE ), respectively. The results showed that the expression levels of some anti‐infectious immunity‐related factors, including interleukin 10 ( IL ‐10), toll‐like receptor 5b ( TLR5b ), and inducible nitric oxide synthase 2a ( iNOS2a ) that were related to A . hydrophila virulent factors under regulation of quorum sensing, were more susceptible to the addition of AiiA B546 . It indicated that AiiA B546 may attenuate the infection of A . hydrophila by interfering with quorum sensing. A . hydrophila was not detected in the enzyme co‐feeding diet group, which might be due to its inability to adhere to the gut with enzyme interference. This study revealed that AiiA B546 as a feed additive might represent a novel biocontrol agent in aquaculture; however, the topic merits further investigations using fish species of commercial interest.