Open AccessEffect of Caffeine Added to the Activating Solution on Sperm Motility of Fresh and Thawed Semen of Pacu, Piaractus mesopotamicus , and Curimba, Prochilodus lineatus
Author(s) -
F. S. Carvalho Aline,
R. Ferreira Machado Monica,
S. Andrade Estefânia,
D. S. Murgas Luis,
G. Zangeronimo Márcio,
C. Barros Thiago,
G. Paula Fernanda
Publication year - 2014
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12091
Subject(s) - semen , pacu , biology , piaractus mesopotamicus , caffeine , sperm , sperm motility , extender , zoology , andrology , botany , anatomy , chemistry , fishery , endocrinology , medicine , organic chemistry , polyurethane
The aim was to evaluate the effect of different concentrations of caffeine added in activating solution over sperm motility in fresh and thawed semen of pacu, Piaractus mesopotamicus , and curimba, Prochilodus lineatus . The activating solutions were prepared with sodium bicarbonate solution of 0.76% ( NaHCO 3 ) and caffeine was added at concentrations of 2.5, 5.0, 10.0, and 20.0 mM . As control, a solution of NaHCO 3 0.76 without caffeine was used. Eight males of pacu and 20 males of curimba were used. Aliquots of 200 μL of semen were diluted in 800 μL extender solution ( DMSO 10% and BTS 5%), placed in 0.5 mL straws and cryopreserved for 7 d in a liquid nitrogen tank. There was a linear increase in sperm motility for fresh semen of pacu, and for curimba fresh and thawed semen ( P < 0.05), due to the increase in the concentration of caffeine. There was a quadratic response for duration of motility for thawed semen of pacu and for fresh semen of curimba ( P < 0.05), respectively. These results indicate that addition of caffeine in the activator solution can improve sperm motility parameters, however, is dependent on the species and concentration used.