Open AccessPhytic Acid‐Induced Inhibition of Digestive Protease and α‐Amylase in Three Indian Major Carps: An in vitro Study
Author(s) -
Khan Argha,
Ghosh Koushik
Publication year - 2013
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12084
Subject(s) - catla , labeo , amylase , protease , biology , phytic acid , digestive enzyme , food science , enzyme , biochemistry , enzyme assay , fish <actinopterygii> , fishery
Antinutritional effects of phytic acid (myoinositol hexaphosphate, IP6 ) on growth and digestibility in fish have been reported. However, specific effect of IP6 on the digestive enzymes in fish has not been addressed. In this study, inhibitory effect of synthetic IP6 (Phytic acid sodium salt, 90% purity) on the activity of the digestive protease and α‐amylase in rohu, Labeo rohita ; catla, Catla catla ; and mrigal, Cirrhinus mrigala has been investigated in vitro . Graded levels (12.5, 25, 50, 100, 150, and 200 µg/ mL ) of IP6 were added to the reaction mixtures containing enzyme extracts and substrate solution in triplicate to detect any change in enzyme activity. Results of the experiment revealed that IP6 significantly inhibit/lower activities of the digestive enzymes in a dose‐dependent manner, as evident from the regression equations ( F values significant at P < 0.001 level). Apparently, irrespective of the fish species studied IP6 ‐induced inhibition of α‐amylase activity was greater than protease activity. Among the three fish species studied, C. mrigala appeared to be more sensitive to IP6 for both α‐amylase and protease activity. Enzyme activity was least affected in C. catla . Results of the study might raise concern while incorporating IP6 rich plant‐derived feed ingredients in aqua feed preparation.