Open AccessCloning and Activity Analysis of Promoter of RanGTPase Gene from Shrimp, Marsupenaeus japonicus
Author(s) -
Han Fang,
Wang ZhiYong
Publication year - 2013
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/jwas.12078
Subject(s) - biology , gene , cloning (programming) , shrimp , promoter , green fluorescent protein , reporter gene , innate immune system , microbiology and biotechnology , gene expression , genetics , immune system , fishery , computer science , programming language
RanGTPases , one superfamily of small G protein family, have been demonstrated to be involved in the innate immune system and they are up‐regulated after pathogen infection. However, the regulation mechanism of RanGTPases remains unclear. In this study, the 5′ flanking region of shrimp RanGTPase gene was cloned and sequenced. To detect the activity of the promoter, pIZ Δ IE / EGFP was modified from pIZ / V5 ‐His by replacing the OPIE2 promoter with this promoter and inserting enhanced green fluorescence protein ( EGFP ) gene downstream. The promoter can drive the expression of reporter EGFP gene in Hi5 insect cells, indicating the promoter has activity. These results extend our previous findings and provide insights into the molecular regulation of RanGTPase gene expression, which will be helpful for shrimp viral disease control.