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Identification and quantification of domestic feline cytochrome P450 transcriptome across multiple tissues
Author(s) -
Visser Marike,
Weber Kristina L.,
Lyons Leslie A.,
Rincon Gonzalo,
Boothe Dawn M.,
Merritt Dawn A.
Publication year - 2019
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/jvp.12708
Subject(s) - gene isoform , transcriptome , biology , cytochrome p450 , complementary dna , gene , computational biology , genome , genetics , cdna library , gene expression , microbiology and biotechnology , biochemistry , enzyme
Understanding of cytochrome P450 ( CYP ) isoform distribution and function in the domestic feline is limited. Only a few studies have defined individual CYP isoforms across metabolically relevant tissues, hampering the ability to predict drug metabolism and potential drug–drug interactions. Using RNA sequencing ( RNA ‐seq), transcriptomes from the 99 Lives Cat Genome Sequencing Initiative databank combined with experimentally acquired whole transcriptome sequencing of healthy, adult male ( n = 2) and female ( n = 2) domestic felines, expression of 42 CYP isoforms were identified in 20 different tissues. Thirty‐seven of these isoforms had not been previously reported in cats. Depending on the tissue, three to twenty‐nine CYP isoform transcripts were expressed. The feline genome annotations did not differentiate CYP 2E1 and 2E2 genes, demonstrating poor annotation for this gene using the reference genome. As the majority of the sequences are based on automated pipelines, complete cDNA sequences for translation into CYP protein sequences could not be determined. This study is the first to identify and characterize 37 additional CYP isoforms in feline tissues, increasing the number of identified CYP from the previously reported seven isoforms to 42 across 20 tissues.